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rabbit anti gpx4  (Huabio Inc)

 
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    Structured Review

    Huabio Inc rabbit anti gpx4
    Rabbit Anti Gpx4, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+gpx4/anti+gpx4/pm42100868-242-24-28
    Average 86 stars, based on 1 article reviews
    rabbit anti gpx4 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers.
    Article Snippet: .. After blocking using 5% fat-free milk in a tris-buffered solution with 0.1% tween-20 (TBST), the membranes were incubated with primary antibodies at 4 °C overnight, including rabbit anti-SIRT1 (#ab76039; 1:2000, Abcam, Cambridge, Massachusetts, USA), rabbit anti-USP28 (#ab188240; 1:2000, Abcam), rabbit anti-NRF2 (#ab313825; 1:1000, Abcam), mouse anti-HO-1 (#ab302671; 1:1000, Abcam), mouse anti-β-actin (#ab8226; 1:10000, Abcam), mouse anti-CHOP (#ab11419; 1:500, Abcam), rabbit antiGRP78 (#ab108615; 1:10000, Abcam), rabbit anti-GPX4 (#ET1706-45; 1:1000, HUABIO, Hangzhou, China), and rabbit anti-ACSL4 (#ET7111-43; 1:1000, HUABIO). .. After washing with TBST, the membranes were incubated with appropriate secondary antibodies (#ab205719/ab205718; 1:10000, Abcam) for 2 h. Subsequently, the bands were visualized by the enhanced chemiluminescence reagent (Beyotime).

    Article Title: USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers
    Article Snippet: .. After blocking using 5% fat-free milk in a tris-buffered solution with 0.1% tween-20 (TBST), the membranes were incubated with primary antibodies at 4 °C overnight, including rabbit anti-SIRT1 (#ab76039; 1:2000, Abcam, Cambridge, Massachusetts, USA), rabbit anti-USP28 (#ab188240; 1:2000, Abcam), rabbit anti-NRF2 (#ab313825; 1:1000, Abcam), mouse anti-HO-1 (#ab302671; 1:1000, Abcam), mouse anti-β-actin (#ab8226; 1:10000, Abcam), mouse anti-CHOP (#ab11419; 1:500, Abcam), rabbit anti-GRP78 (#ab108615; 1:10000, Abcam), rabbit anti-GPX4 (#ET1706-45; 1:1000, HUABIO, Hangzhou, China), and rabbit anti-ACSL4 (#ET7111-43; 1:1000, HUABIO). .. After washing with TBST, the membranes were incubated with appropriate secondary antibodies (#ab205719/ab205718; 1:10000, Abcam) for 2 h. Subsequently, the bands were visualized by the enhanced chemiluminescence reagent (Beyotime).

    Incubation:

    Article Title: USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers.
    Article Snippet: .. After blocking using 5% fat-free milk in a tris-buffered solution with 0.1% tween-20 (TBST), the membranes were incubated with primary antibodies at 4 °C overnight, including rabbit anti-SIRT1 (#ab76039; 1:2000, Abcam, Cambridge, Massachusetts, USA), rabbit anti-USP28 (#ab188240; 1:2000, Abcam), rabbit anti-NRF2 (#ab313825; 1:1000, Abcam), mouse anti-HO-1 (#ab302671; 1:1000, Abcam), mouse anti-β-actin (#ab8226; 1:10000, Abcam), mouse anti-CHOP (#ab11419; 1:500, Abcam), rabbit antiGRP78 (#ab108615; 1:10000, Abcam), rabbit anti-GPX4 (#ET1706-45; 1:1000, HUABIO, Hangzhou, China), and rabbit anti-ACSL4 (#ET7111-43; 1:1000, HUABIO). .. After washing with TBST, the membranes were incubated with appropriate secondary antibodies (#ab205719/ab205718; 1:10000, Abcam) for 2 h. Subsequently, the bands were visualized by the enhanced chemiluminescence reagent (Beyotime).

    Article Title: USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers
    Article Snippet: .. After blocking using 5% fat-free milk in a tris-buffered solution with 0.1% tween-20 (TBST), the membranes were incubated with primary antibodies at 4 °C overnight, including rabbit anti-SIRT1 (#ab76039; 1:2000, Abcam, Cambridge, Massachusetts, USA), rabbit anti-USP28 (#ab188240; 1:2000, Abcam), rabbit anti-NRF2 (#ab313825; 1:1000, Abcam), mouse anti-HO-1 (#ab302671; 1:1000, Abcam), mouse anti-β-actin (#ab8226; 1:10000, Abcam), mouse anti-CHOP (#ab11419; 1:500, Abcam), rabbit anti-GRP78 (#ab108615; 1:10000, Abcam), rabbit anti-GPX4 (#ET1706-45; 1:1000, HUABIO, Hangzhou, China), and rabbit anti-ACSL4 (#ET7111-43; 1:1000, HUABIO). .. After washing with TBST, the membranes were incubated with appropriate secondary antibodies (#ab205719/ab205718; 1:10000, Abcam) for 2 h. Subsequently, the bands were visualized by the enhanced chemiluminescence reagent (Beyotime).



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    Image Search Results


    Alcohol exposure triggers ferroptosis, which undermines cell growth. A . An equal number of HEEC or Het1A cells was seeded in 6-well plates and incubated with the complete medium (control) or the medium containing 5.0% ethanol (EtOH) for 5 min, 30 min, 2 h, 6 h, or 12 h. Protein was extracted and analyzed by Western blotting for GPX4. GAPDH was used as a loading control. B . Quantitative analyses of GPX4 expression against GAPDH in HEEC and Het1A cells. * indicates a significant change compared to the control. C . An equal number of HEEC cells was seeded on coverslips and incubated with complete medium (control) or medium containing 5.0% ethanol for 5 min, 30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for iron. Scale bar = 10 μm. D . An equal number of HEEC or Het1A cells was seeded on coverslips and incubated with complete medium (control) or medium containing 5.0% ethanol for 5 min, 30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for iron. Arrows point to the iron-containing cells. Scale bar = 10 μm. E . Percentage of iron-containing cells in the total number of cells (%). * indicates a significant change compared to the control

    Journal: BMC Molecular and Cell Biology

    Article Title: Alcohol exposure induces ferroptosis-dominated programmed cell death in esophageal epithelial cells

    doi: 10.1186/s12860-026-00589-5

    Figure Lengend Snippet: Alcohol exposure triggers ferroptosis, which undermines cell growth. A . An equal number of HEEC or Het1A cells was seeded in 6-well plates and incubated with the complete medium (control) or the medium containing 5.0% ethanol (EtOH) for 5 min, 30 min, 2 h, 6 h, or 12 h. Protein was extracted and analyzed by Western blotting for GPX4. GAPDH was used as a loading control. B . Quantitative analyses of GPX4 expression against GAPDH in HEEC and Het1A cells. * indicates a significant change compared to the control. C . An equal number of HEEC cells was seeded on coverslips and incubated with complete medium (control) or medium containing 5.0% ethanol for 5 min, 30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for iron. Scale bar = 10 μm. D . An equal number of HEEC or Het1A cells was seeded on coverslips and incubated with complete medium (control) or medium containing 5.0% ethanol for 5 min, 30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for iron. Arrows point to the iron-containing cells. Scale bar = 10 μm. E . Percentage of iron-containing cells in the total number of cells (%). * indicates a significant change compared to the control

    Article Snippet: The following primary antibodies were used: Beclin-1 (Origene, #TA502643), PCNA, CASP1 (Abcam, #ab179515), CASP3 (Santa Cruz Biotechnology, #sc-271759), CASP8 (Origene, #TA374288), CASP9 (Origene, #TA4227045), endoG, GPX4 (Origene, #TA423164M), GSDMD, IL-1β (Santa Cruz Biotechnology, #sc-12742), MLKL, phosphor-MLKL (Abcam, #ab196436), SLC7A11 (Origene, #TA423232), LC3B, BAX, GAPDH (OriGene, #TA800894), and β-actin (Santa Cruz Biotechnology, Inc. #sc-69879).

    Techniques: Incubation, Control, Western Blot, Expressing, Staining